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Samples must be collected within two to four hours post-administration to capture measurable concentrations, and immediate processing (centrifugation, plasma separation, freezing at 80C) is essential to prevent ex vivo degradation

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2.6 Preparation of aortic tissue After euthanizing mice from different groups using gas anesthesia, the thoracic aorta was surgically excised

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showed that during AD onset phase, patients had reduced circulating levels of melatonin and beta-endorphin, despite higher nocturnal levels of both hormones